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KMID : 0364820120480030187
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Korean Journal of Microbiology
2012 Volume.48 No. 3 p.187 ~ p.191
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Heavy Metal Detection and Removal in Artificial Wastewater Using Two-Component System Based Recombinant Bacteria
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Sambandam Ravikumar
Hong Soon-ho
Yoo Ik-Keun
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Abstract
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Two-component system (TCS)-based bacterial zinc and copper biosensors, in which green fluorescent
protein (GFP) is expressed under the control of zraP and cusC promoter in ZraS/R and CusS/R TCS, were
evaluated in artificial wastewater. Bacterial biosensors developed in this study efficiently expressed GFP by
the recognition of Zn2+ and Cu2+ in artificial wastewater. Secondly, TCS-based zinc and copper removing
bacteria with the peptide displayed on cell surface were examined in artificial wastewater. Zinc and copper
removing bacteria expressed the peptide as a fusion protein such as OmpC-ZBP (zinc binding peptide) and
OmpC-CBP (copper binding peptide) on the cell surface when sensing exogenous Zn2+ and Cu2+ through
ZraS/R and CusS/R TCS. The recombinant cell expressing metal-adsorbing peptide could efficiently remove
copper and zinc (15 and 18 mg/g dry cell weight, respectively) in artificial wastewater. Therefore, it was
demonstrated that the TCS-based recombinant cell for the recognition or removal of heavy metal functions
well in artificial wastewater environment.
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KEYWORD
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Escherichia coli, artificial wastewater, biosensor, copper, zinc
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